microscale protein labeling kit alexa fluor® 647 (Thermo Fisher)
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microscale protein labeling kit alexa fluor® 647
Microscale Protein Labeling Kit Alexa Fluor® 647, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alexa+fluor+647+protein+labeling+kit/alexa+fluor+488+protein+labeling+kit/pmc11194514__pnas__2316615121__sapp-285-12-16
Average 90 stars, based on 1 article reviews
Microscale Protein Labeling Kit Alexa Fluor® 647, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alexa+fluor+647+protein+labeling+kit/alexa+fluor+488+protein+labeling+kit/pmc11194514__pnas__2316615121__sapp-285-12-16
Average 90 stars, based on 1 article reviews
microscale protein labeling kit alexa fluor® 647 - by Bioz Stars,
2026-10
90/100 stars
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Labeling:Article Title: Ex vivo imaging reveals the spatiotemporal control of ovulation Article Snippet: .. The fluorescent dextran (Thermo Fisher, D7144) was labelled using the Article Title: DET1 dynamics underlie cooperative ubiquitination by CRL4 DET1-COP1 complexes Article Snippet: .. Proteins required for fluorescence labeling were dialyzed into phosphate-buffered saline (PBS) (pH 8.0) and labeled using the Article Title: Nucleic-acid-induced ZCCHC3 condensation promotes broad innate immune responses. Article Snippet: Article Article Title: DET1 dynamics underlie cooperative ubiquitination by CRL4 DET1-COP1 complexes. Article Snippet: .. Proteins required for fluorescence labeling were dialyzed into phosphate- buffered saline (PBS) (pH 8.0) and labeled using the Article Title: X-Ray Photon Correlation Spectroscopy, Microscopy, and Fluorescence Recovery After Photobleaching to Study Phase Separation and Liquid-to-Solid Transition of Prion Protein Condensates Article Snippet: Polyethylene glycol (PEG) 4000 (Sigma-Aldrich, catalog number: 81240) 11. .. Article Title: Eliciting a single amino acid change by vaccination generates antibody protection against group 1 and group 2 influenza A viruses Article Snippet: .. Article Title: Method for determining the in vivo interaction mode Article Snippet: .. CEACAM5 Cell Surface Binding Assay 1×105 gastric adenocarcinoma cells cultured in RPMI1640, 20% fetal bovine serum (FBS), 1× GIBCO GlutaMax (Thermo Fischer Scientific, Dreieich, Germany) were washed twice with PBS, 5% FBS, resuspended in PBS, 5% FBS and incubated with the anti-CEA antibody, a purified Fab fragment obtained by papain digestion of said anti-CEA antibody, said antibody digested with lysine-gingipain of Porphyromonas gingivalis and negative controls (an antibody binding to a non-related target, lysine-gingipain of Porphyromonas gingivalis only) for one hour at 4° C. Bound antibodies/Fab fragments were detected using a mouse anti-human kappa light chain antibody (150 μg/mL) labeled using the Fluorescence:Article Title: DET1 dynamics underlie cooperative ubiquitination by CRL4 DET1-COP1 complexes Article Snippet: .. Proteins required for fluorescence labeling were dialyzed into phosphate-buffered saline (PBS) (pH 8.0) and labeled using the Article Title: DET1 dynamics underlie cooperative ubiquitination by CRL4 DET1-COP1 complexes. Article Snippet: .. Proteins required for fluorescence labeling were dialyzed into phosphate- buffered saline (PBS) (pH 8.0) and labeled using the Saline:Article Title: DET1 dynamics underlie cooperative ubiquitination by CRL4 DET1-COP1 complexes Article Snippet: .. Proteins required for fluorescence labeling were dialyzed into phosphate-buffered saline (PBS) (pH 8.0) and labeled using the Article Title: DET1 dynamics underlie cooperative ubiquitination by CRL4 DET1-COP1 complexes. Article Snippet: .. Proteins required for fluorescence labeling were dialyzed into phosphate- buffered saline (PBS) (pH 8.0) and labeled using the Purification:Article Title: Nucleic-acid-induced ZCCHC3 condensation promotes broad innate immune responses. Article Snippet: Article Article Title: Method for determining the in vivo interaction mode Article Snippet: .. CEACAM5 Cell Surface Binding Assay 1×105 gastric adenocarcinoma cells cultured in RPMI1640, 20% fetal bovine serum (FBS), 1× GIBCO GlutaMax (Thermo Fischer Scientific, Dreieich, Germany) were washed twice with PBS, 5% FBS, resuspended in PBS, 5% FBS and incubated with the anti-CEA antibody, a purified Fab fragment obtained by papain digestion of said anti-CEA antibody, said antibody digested with lysine-gingipain of Porphyromonas gingivalis and negative controls (an antibody binding to a non-related target, lysine-gingipain of Porphyromonas gingivalis only) for one hour at 4° C. Bound antibodies/Fab fragments were detected using a mouse anti-human kappa light chain antibody (150 μg/mL) labeled using the other:Article Title: Enhanced hammerhead ribozyme turnover rates: reevaluating therapeutic space for small catalytic RNAs Article Snippet: After the bicistronic plasmid-transfected cells were imaged for 1179 mCherry, the 6 well plate was washed 3X in PBS and fixed for 10 min in ice cold methanol, 1180 then washed 3X in PBS and the hRHO Alexa 647 monoclonal 1D4 antibody (558 ng/ml) is 1181 added for 1 hour at Binding Assay:Article Title: Method for determining the in vivo interaction mode Article Snippet: .. CEACAM5 Cell Surface Binding Assay 1×105 gastric adenocarcinoma cells cultured in RPMI1640, 20% fetal bovine serum (FBS), 1× GIBCO GlutaMax (Thermo Fischer Scientific, Dreieich, Germany) were washed twice with PBS, 5% FBS, resuspended in PBS, 5% FBS and incubated with the anti-CEA antibody, a purified Fab fragment obtained by papain digestion of said anti-CEA antibody, said antibody digested with lysine-gingipain of Porphyromonas gingivalis and negative controls (an antibody binding to a non-related target, lysine-gingipain of Porphyromonas gingivalis only) for one hour at 4° C. Bound antibodies/Fab fragments were detected using a mouse anti-human kappa light chain antibody (150 μg/mL) labeled using the Cell Culture:Article Title: Method for determining the in vivo interaction mode Article Snippet: .. CEACAM5 Cell Surface Binding Assay 1×105 gastric adenocarcinoma cells cultured in RPMI1640, 20% fetal bovine serum (FBS), 1× GIBCO GlutaMax (Thermo Fischer Scientific, Dreieich, Germany) were washed twice with PBS, 5% FBS, resuspended in PBS, 5% FBS and incubated with the anti-CEA antibody, a purified Fab fragment obtained by papain digestion of said anti-CEA antibody, said antibody digested with lysine-gingipain of Porphyromonas gingivalis and negative controls (an antibody binding to a non-related target, lysine-gingipain of Porphyromonas gingivalis only) for one hour at 4° C. Bound antibodies/Fab fragments were detected using a mouse anti-human kappa light chain antibody (150 μg/mL) labeled using the Incubation:Article Title: Method for determining the in vivo interaction mode Article Snippet: .. CEACAM5 Cell Surface Binding Assay 1×105 gastric adenocarcinoma cells cultured in RPMI1640, 20% fetal bovine serum (FBS), 1× GIBCO GlutaMax (Thermo Fischer Scientific, Dreieich, Germany) were washed twice with PBS, 5% FBS, resuspended in PBS, 5% FBS and incubated with the anti-CEA antibody, a purified Fab fragment obtained by papain digestion of said anti-CEA antibody, said antibody digested with lysine-gingipain of Porphyromonas gingivalis and negative controls (an antibody binding to a non-related target, lysine-gingipain of Porphyromonas gingivalis only) for one hour at 4° C. Bound antibodies/Fab fragments were detected using a mouse anti-human kappa light chain antibody (150 μg/mL) labeled using the |
![(A) Imaging chamber for FRAP experiment as described in [23]. To keep the buffer composition and protein concentration unchanged during FRAP acquisition, it is necessary to prepare this humid chamber as evaporation can occur during acquisition. After the addition of ultrapure H 2 O in the tissue surrounding the coverslip, 3–5 μL of each sample is loaded, and the lid is immediately closed. (B) FRAP recovery of an in vitro prion protein (PrP) condensate formed with 10 μM recombinant PrP (spiked with 0.1% <t>Alexa</t> Fluor <t>647–labeled</t> rPrP) upon addition of 80 μM CuCl 2 . The recovery curve of a single condensate is shown after background correction and normalization (Section M). Inset show a PrP:Cu 2+ condensate pre-bleaching, immediately after bleaching, and after fluorescence recovery. The experiment was performed 15 min after sample preparation.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1588/pmc12021588/pmc12021588__BioProtoc-15-8-5277-g005.jpg)